
Our client’s requirement
The client worked in the area of in-silico sensitivity and specificity analysis of probes and primers for SARS-CoV2 molecular diagnostic assays. They aimed to obtain the tabular output of gene variants and in-depth variant prevalence information from non-redundant and filtered sequence alignments.
Our approach
-
We retrieved the SARS-CoV2 multiple sequence alignment from reliable sources such as Nextstrain/Genbank.
-
We conducted an in-depth analysis of the prevalence of each variant in the two genes of interest.
-
Developed a workflow tailored explicitly for extracting two specified gene sequences.