Accelerating the Development of a Rapid Detection NAAT Assay for SARS-CoV2


Our client’s requirement

The client worked in the area of in-silico sensitivity and specificity analysis of probes and primers for SARS-CoV2 molecular diagnostic assays. They aimed to obtain the tabular output of gene variants and in-depth variant prevalence information from non-redundant and filtered sequence alignments.

Our approach

We implemented the following key activities to develop the rapid detection NAAT assay faster.
  1. We retrieved the SARS-CoV2 multiple sequence alignment from reliable sources such as Nextstrain/Genbank.
  2. We conducted an in-depth analysis of the prevalence of each variant in the two genes of interest.
  3. Developed a workflow tailored explicitly for extracting two specified gene sequences.
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